doc. RNDr. Ivo Rudolf, Ph.D.

Position: head of the facility
Research topics: eco-epidemiology of microbial pathogens
Department: External research facility Valtice
Phone: +420 519 352 961

Research interests

  • isolation and identification of microorganisms,
  • research on emerging infections,
  • molecular diagnostics of zoonotic pathogens in haematophagous arthropods and vertebrate hosts.

Finished Projects

Diversity of cultivable microorganisms of ixodid ticks, recognized vectors of vertebrate pathogens



Blažejová H., Šebesta O., Rettich F., Mendel J., Čabanová V., Miterpáková M., Betášová L., Peško J., Hubálek Z., Kampen H., Rudolf I.: Cryptic species Anopheles daciae (Diptera: Culicidae) found in the Czech Republic and Slovakia, Parasitology Research 117 (2018) 315-321.

Čabanová V., Miterpáková M., Valentová D., Blažejová H., Rudolf I., Stloukal E., Hurníková Z., Dzidová M.: Urbanization impact on mosquito community and the transmission potential of filarial infection in central Europe, Parasites & Vectors 11 (2018) .

Haviernik J., Štefánik M., Fojtíková M., Kali S., Tordo N., Rudolf I., Hubálek Z., Eyer L., Růžek D.: Arbidol (Umifenovir): A broad-spectrum antiviral drug that inhibits medically important arthropod-borne flaviviruses, Viruses 10 (2018) .

Ros R., Andreo V., Tagliapietra V., Baráková I., Arnoldi D., Hauffe H. C., Manica M., Rosso F., Blaňarová L., Bona M., Derdáková M., Hamšíková Z., Kazimírová M., Kraljik J., Kocianová E., Mahríková L., Minichová L., Mošanský L., Slovák M., Stanko M., Špitalská E., Ducheyne E., Neteler M., Hubálek Z., Rudolf I., Venclíková K., Silaghi C., Overzier E., Farkas R., Földvári G., Hornok S., Takács N., Rizzoli A.: Effect of climate and land use on the spatio-temporal variability of tick-borne bacteria in Europe, International Journal of Environmental Research and Public Health 15 (2018) .

Rudolf I., Blažejová H., Šebesta O., Mendel J., Peško J., Betášová L., Straková P., Šikutová S., Hubálek Z.: West Nile virus (linie 2) v komárech na jižní Moravě – očekávání prvních autochtonních lidských případů, Epidemiologie, Mikrobiologie, Imunologie (2018) 44-46.

Rudolf I., Blažejová H., Straková P., Šebesta O., Peško J., Mendel J., Šikutová S., Hubálek Z., Kampen H., Schaffner F.: The invasive Asian tiger mosquito Aedes albopictus (Diptera: Culicidae) in the Czech Republic: Repetitive introduction events highlight the need for extended entomological surveillance, Acta Tropica 185 (2018) 239-241.

Straková P., Kubánková M., Vašíčková P., Juřicová Z., Rudolf I., Hubálek Z.: Hepatitis E virus in archived sera from wild boars (Sus scrofa), Czech Republic, Transboundary and Emerging Diseases (2018) .


Hubálek Z., Rudolf I.: Francisella tularensis prevalence and load in Dermacentor reticulatus ticks in an endemic area in Central Europe, Medical and Veterinary Entomology 31 (2017) 234-239.

Hubálek Z., Juřicová Z., Straková P., Blažejová H., Betášová L., Rudolf I.: Serological survey for West Nile virus in wild artiodactyls, Southern Moravia (Czech Republic), Vector-Borne and Zoonotic Diseases 17 (2017) 654-657.

Rudolf I., Betášová L., Blažejová H., Venclíková K., Straková P., Šebesta O., Mendel J., Bakonyi T., Schaffner F., Nowotny N., Hubálek Z.: West Nile virus in overwintering mosquitoes, central Europe, Parasites & Vectors 10 (2017) .


Bakonyi T., Gaydon G. K., Schwing R., Vogl W., Häbich A.-C., Thaller D., Weissenböck H., Rudolf I., Hubálek Z., Nowotny N.: Chronic West Nile virus infection in kea (Nestor notabilis), Veterinary Microbiology 183 (2016) 135-139.

Duscher G. G., Hodžić A., Weiler M., Vaux A. G. C., Rudolf I., Sixl W., Medlock J. M., Versteirt V., Hubálek Z.: First report of Rickettsia raoultii in field collected Dermacentor reticulatus ticks from Austria, Ticks and Tick-borne Diseases 7 (2016) 720-722.

Hamšíková Z., Silaghi C., Rudolf I., Venclíková K., Mahríková L., Slovák M., Mendel J., Blažejová H., Berthová L., Kocianová E., Hubálek Z., Schnittger L., Kazimírová M.: Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic, Parasitology Research 115 (2016) 3897-3904.

Rudolf I., Betášová L., Bischof V., Venclíková K., Blažejová H., Mendel J., Hubálek Z., Kosoy M.: Molecular survey of arthropod-borne pathogens in sheep keds (Melophagus ovinus), Central Europe, Parasitology Research 115 (2016) 3679-3682.

Rudolf I., Venclíková K., Blažejová H., Betášová L., Mendel J., Hubálek Z., Parola P.: First report of Rickettsia raoultii and Rickettsia helvetica in Dermacentor reticulatus ticks from the Czech Republic, Ticks and Tick-borne Diseases 7 (2016) 1222-1224.

Venclíková K., Mendel J., Betášová L., Blažejová H., Jedličková P., Straková P., Hubálek Z., Rudolf I.: Neglected tick-borne pathogens in the Czech Republic, 2011–2014, Ticks and Tick-borne Diseases 7 (2016) 107-112.


Kolodziejek J., Seidel B., Jungbauer C., Dimmel K., Kolodziejek M., Rudolf I., Hubálek Z., Allerberger F., Nowotny N.: West Nile Virus positive blood donation and subsequent entomological investigation, Austria, 2014, PLoS ONE 10 (2015) .

Rudolf I., Šebesta O., Straková P., Betášová L., Blažejová H., Venclíková K., Seidel B., Tóth S., Hubálek Z., Schaffner F.: Overwintering of Uranotaenia unguiculata adult females in central Europe: a possible way of persistence of the putative new lineage of West Nile virus?, Journal of the American Mosquito Control Association 31 (2015) 364-365.

Rudolf I., Bakonyi T., Šebesta O., Mendel J., Peško J., Betášová L., Blažejová H., Venclíková K., Straková P., Nowotny N., Hubálek Z.: Co-circulation of Usutu virus and West Nile virus in a reed bed ecosystem, Parasites & Vectors 8 (2015) 520.

Straková P., Šikutová S., Jedličková P., Sitko J., Rudolf I., Hubálek Z.: The common coot as sentinel species for the presence of West Nile and Usutu flaviviruses in Central Europe, Research in Veterinary Science 102 (2015) 159-161.

Venclíková K., Mendel J., Betášová L., Hubálek Z., Rudolf I.: First evidence of Babesia venatorum and Babesia capreoli in questing Ixodes ricinus ticks in the Czech Republic, Annals of Agricultural and Environmental Medicine 22 (2015) 212-214.

Vrbovská V., Chalupa P., Straková P., Hubálek Z., Rudolf I.: Onemocnění člověka způsobená hantaviry – stále opomíjené zoonózy?, Epidemiologie, Mikrobiologie, Imunologie (2015) 188-196.


Hubálek Z., Rudolf I., Nowotny N.: Arboviruses pathogenic for domestic and wild animals, Advances in Virus Research 89 (2014) 201-275.

Hubálek Z., Šebesta O., Peško J., Betášová L., Blažejová H., Venclíková K., Rudolf I.: Isolation of Tahyna virus (California Encephalitis Group) from Anopheles hyrcanus (Diptera, Culicidae), a mosquito species new to, and expanding in, Central Europe, Journal of Medical Entomology 51 (2014) 1264-1267.

Hubálek Z., Rudolf I., Čapek M., Bakonyi T., Betášová L., Nowotny N.: Usutu Virus in Blackbirds (Turdus merula), Czech Republic, 2011-2012, Transboundary and Emerging Diseases 61 (2014) 273-276.

Pachler K., Lebl K., Berer D., Rudolf I., Hubálek Z., Nowotny N.: Putative New West Nile virus lineage in Uranotaenia unguiculata mosquitoes, Austria, 2013, Emerging Infectious Diseases 20 (2014) 2119-2122.

Rizzoli A., Silaghi C., Obiegala A., Rudolf I., Hubálek Z., Földvári G., Plantard O., Vayssier-Taussat M., Bonnet S., Špitalská E., Kazimírová M.: Ixodes ricinus and its transmitted pathogens in urban and peri-urban areas in Europe: new hazards and relevance for public health, Frontiers in Public Health 2 (2014) .

Rudolf I., Šebesta O., Mendel J., Betášová L., Bocková E., Jedličková P., Venclíková K., Blažejová H., Šikutová S., Hubálek Z.: Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic, Parasitology Research 113 (2014) 4663-4667.

Rudolf I., Bakonyi T., Šebesta O., Mendel J., Peško J., Betášová L., Blažejová H., Venclíková K., Straková P., Nowotny N., Hubálek Z.: West Nile virus lineage 2 isolated from Culex modestus mosquitoes in the Czech Republic, 2013: expansion of the European WNV endemic area to the North?, Eurosurveillance 19 (2014) .

Straková P., Rudolf I., Pavlis O., Hubálek Z.: The use of immunoenzymatic method for detection of antibodies against zoonotic diseases in Czech soldiers returning from Afghanistan, Military Medical Science Letters 83 (2014) 67-72.

Straková P., Kříž B., Rudolf I., Hubálek Z.: Seroprevalence study of hepatitis E virus infection in two districts of the Czech Republic, Epidemiologie, Mikrobiologie, Imunologie (2014) 92-94.

Šikutová S., Buňková L., Krejčí E., Halouzka J., Sanogo Y. O., Rudolf I.: Selected phenotypic features of BR91, a unique spirochaetal strainisolated from the Culex pipiens mosquito, Microbiological Research 169 (2014) 348-352.

Venclíková K., Rudolf I., Mendel J., Betášová L., Hubálek Z.: Rickettsiae in questing Ixodes ricinus ticks in the Czech Republic, Ticks and Tick-borne Diseases 5 (2014) 135-138.

Venclíková K., Betášová L., Šikutová S., Jedličková P., Hubálek Z., Rudolf I.: Human pathogenic borreliae in Ixodes ricinus ticks in natural and urban ecosystem (Czech Republic), Acta Parasitologica 59 (2014) 717-720.


Bakonyi T., Kolodziejek J., Rudolf I., Berčič R., Nowotny N., Hubálek Z.: Partial genetic characterization of Sedlec virus (Orthobunyavirus, Bunyaviridae), Infection, Genetics and Evolution (2013) 244-249.

Bocková E., Rudolf I., Kočišová A., Betášová L., Venclíková K., Mendel J., Hubálek Z.: Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia, Parasitology Research 112 (2013) 3465-3470.

Hubálek Z., Ludvíková E., Jahn P., Treml F., Rudolf I., Svobodová P., Šikutová S., Betášová L., Bíreš J., Mojžíš M., Tinák M., Boldižár M., Citsoňová G., Staššíková Z.: West Nile virus equine serosurvey in the Czech and Slovak Republics, Vector-Borne and Zoonotic Diseases 13 (2013) 733-738.

Sedláček I., Holochová P., Mašlaňová I., Kosina M., Spröer C., Bryndová H., Vandamme P., Rudolf I., Hubálek Z., Švec P.: Enterococcus ureilyticus sp. nov. and Enterococcus rotai sp. nov., two urease-producing enterococci from the environment, International Journal of Systematic and Evolutionary Microbiology 63 (2013) 502-510.


Hubálek Z., Rudolf I.: Tick-borne viruses in Europe, Parasitology Research 111 (2012) 9-36.

Konvalinová J., Rudolf I., Šikutová S., Hubálek Z., Svobodová V., Svoboda M.: Contribution to canine babesiosis in the Czech Republic, Acta veterinaria Brno 81 (2012) 91-95.

Šebesta O., Rudolf I., Betášová L., Peško J., Hubálek Z.: An invasive mosquito species Aedes albopictus found in the Czech Republic, 2012, Eurosurveillance 17 (2012) 20301.


Hubálek Z., Rudolf I., Bakonyi T., Kazdová K., Halouzka J., Šebesta O., Šikutová S., Juřicová Z., Nowotny N.: Mosquito (Diptera: Culicidae) surveillance for arboviruses in an area endemic for West Nile (Lineage Rabensburg) and Ťahyňa viruses in central Europe, Journal of Medical Entomology 47 (2010) 466-472.

Rudolf I., Šikutová S., Kopecký J., Hubálek Z.: Salivary gland extract from engorged Ixodes ricinus (Acari: Ixodidae) stimulates in vitro growth of Borrelia burgdorferi sensu lato, Journal of Basic Microbiology 50 (2010) 294-298.

Šebesta O., Halouzka J., Hubálek Z., Juřicová Z., Rudolf I., Šikutová S., Svobodová P., Reiter P.: Mosquito (Diptera: Culicidae) fauna in an area endemic for West Nile virus, Journal of Vector Ecology 35 (2010) 156-162.

Šikutová S., Halouzka J., Mendel J., Knoz J., Rudolf I.: Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic, Journal of Vector Ecology 35 (2010) 50-55.


Jarošová V., Rudolf I., Halouzka J., Hubálek Z.: Borrelia burgdorferi s.l. v klíšťatech na ostravských haldách, Epidemiologie, Mikrobiologie, Imunologie (2009) 90-97.

Rudolf I., Mendel J., Šikutová S., Švec P., Masaříková J., Nováková D., Buňková L., Sedláček I., Hubálek Z.: 16S rRNA gene-based identification of cultured bacterial flora from host-seeking Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna ticks, vectors of vertebrate pathogens, Folia Microbiologica 54 (2009) 419-428.

Šebesta O., Rettich F., Minář J., Halouzka J., Hubálek Z., Juřicová Z., Rudolf I., Šikutová S., Gelbič I., Reiter P.: Presence of the mosquito Anopheles hyrcanus in South Moravia, Czech Republic, Medical and Veterinary Entomology 23 (2009) 284-286.

Šikutová S., Hornok S., Hubálek Z., Doležálková I., Juřicová Z., Rudolf I.: Serological survey of domestic animals for tick-borne encephalitis and Bhanja viruses in northeastern Hungary, Veterinary Microbiology 135 (2009) 267-271.


Buňková L., Švec P., Halouzka J., Rudolf I., Němec M.: Ribotyping and whole-cell protein analysis of spirochetes isolated from arthropods in the Czech Republic, Annals of Agricultural and Environmental Medicine 15 (2008) 225-230.

Hubálek Z., Wegner E., Halouzka J., Tryjanowski P., Jerzak L., Šikutová S., Rudolf I., Kruszewicz A. G., Jaworski Z., Włodarczyk R.: Serologic survey of potential vertebrate hosts for West Nile Virus in Poland, Viral Immunology 21 (2008) 247-254.

Hubálek Z., Halouzka J., Juřicová Z., Šikutová S., Rudolf I., Honza M., Janková J., Chytil J., Marec F., Sitko J.: Serologic survey of birds for West Nile Flavivirus in Southern Moravia (Czech Republic), Vector-Borne and Zoonotic Diseases 8 (2008) 659-666.

Meister T., Lussy H., Bakonyi T., Šikutová S., Rudolf I., Vogl W., Winkler H., Frey H., Hubálek Z., Nowotny N., Weissenböck H.: Serological evidence of continuing high Usutu virus (Flaviviridae) activity and establishment of herd immunity in wild birds in Austria, Veterinary Microbiology 127 (2008) 237-248.

Rudolf I., Hubálek Z., Šikutová S., Švec P.: Opomíjené virové infekce přenášené hematofágními členovci v České republice, Epidemiologie, Mikrobiologie, Imunologie (2008) 80-89.